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29 Jun 2019 The enzyme CGTase has proved an exceptional capability to glucosylate compounds of (CGT-SL) was from Amano Enzyme Inc. (Nagoya,.

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CIs by the action of CGTase. The reaction condition for synthesis of branched CI was as follows; 4.5 g of maltose, 1.0 g of CI-7, 10 ml of 100 mM acetate buffer (pH 5.5), 85 ml of deionized water, and 3 ml of CGTase solution (300 U/ml) were mixed and incubated at 40t for 24 h.

Ciclomaltodextrin. Glucanotransferasa. (CGTase). Feruloil Esterasa.

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Soluble starch, which was used as the substrate for the CGTase activityassay,waspurchasedfromMerck(Germany). -CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA). All other chemicals used were of reagent grade. 2.2. the Preparation and characterization of CGTase In our work, the CGTase from Thermoanaerobacter sp. (Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with increasing concentration, indicating the formation of the so‐called analogous series, Figure 2 The distance of CGTase (EC 2.4.1.19) from Bacillus macerans was purchased from the needle tip from the substrate was varied in the range of 10 Amano Enzyme, Inc. (Japan), and was used without further purifi- to 25 cm.

The optimum Tesque, Amano Enzyme, or Sigma-Aldrich. PaGT-3 was expressed and purified as described previously [7, 8].

Bacillus macerans CGTase, here called Amano, (EC.2.4.1.19) was kindly provided by Amano enzyme Europe Ltd. (Milton Keynes, UK) and Thermoanaerobacter sp. ATCC 53627 CGTase (Toruzyme 3.0L,) from Novozymes (Bagsvaerd, Denmark). These enzymes have been

CGTase was purchased from Amano Enzyme Europe Ltd (Milton Keynes, UK) and Toruzyme 3.0L was obtained from Novozyme A/S (Bagsvaerd, Denmark). Dodecyl-β-maltooctaoside was produced in-house as described previously (Svensson et al. 2009a).

Cgtase amano

例えば、ブレビバクテリウム（Brevibacterium）属の生産するγ-CGTaseが用いられる。 【選択図】なし JP2005312464A - 配糖体及び糖転移生成物の製造法 - Google Patents 配糖体及び糖 2005-07-25 Application filed by Amano Enzyme Inc,

Cgtase amano

Because time-consuming and expensive purification procedures hinder the widespread application of single-ingredient cyclodextrins, enzymes with enhanced specificity are needed. In this study, we tested the hypothesis that the α Amano Enzyme, Inc. (Japan), and was used without further purifi-cation. Soluble starch, which was used as the substrate for the CGTase activityassay,waspurchasedfromMerck(Germany). -CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA). All other chemicals used were of reagent grade. 2.2. the Preparation and characterization of CGTase Synthesis of β-maltosides of resveratrol.

Cgtase amano

Närproducerade, ekologiska råvaror och viner av högsta kvalité brinner vi för. Färsk gös direkt från Hjälmaren, lokalt plockad svamp och oljor från Julita är bara ett par exempel. A bacterium that secretes maltooligosaccharide-forming amylase in a medium containing 12.5% (vol/vol) dimethylsulfoxide (DMSO) was isolated and identified as Brachybacterium sp. strain LB25. The amylase of the strain was purified from the culture supernatant, and its molecular mass was 60 kDa.
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In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with We are grateful for the kind gift of the CGTase enzyme isolated from Bacillus macerans provided by Amano Enzyme Inc., Nagoya, Japan. We thank the Villum Foundation and Carlsberg Foundation for financial support. 7KLV (OHFWURQLF6XSSOHPHQWDU\0DWHULDO (6, IRU&KHP&RPP MRXUQDOLV 7KH 5R\DO 6RFLHW\RI&KHPLVWU\ Since 1951 Amano has been providing environmental solutions that improve the workplace.

-CD was purchased from Sigma-Aldrich (M) Sdn. Bhd. (USA). All other chemicals used were of reagent grade.
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CIs by the action of CGTase. The reaction condition for synthesis of branched CI was as follows; 4.5 g of maltose, 1.0 g of CI-7, 10 ml of 100 mM acetate buffer (pH 5.5), 85 ml of deionized water, and 3 ml of CGTase solution (300 U/ml) were mixed and incubated at 40t for 24 h.

(Toruzyme 3.0 L) gave a higher yield than that from Bacillus macerans (CGTase Amano). In contrast with the CGTase from Bacillus macerans (the HPLC chromatogram showed four different compounds with increasing concentration, indicating the formation of the so‐called analogous series, Figure 2 glycosyltransferase (CGTase) gave rise to a significant formation of glycosylated products.


Grignard reagents are strong electrophiles

cyclomaltodextrin glycosyltransferase; konchizaimu; α-1,4-glucan 4- glycosyltransferase, cyclizing; BMA; CGTase; neutral-cyclodextrin glycosyltransferase; 

Dodecyl-β-maltooctaoside was produced in-house as described previously (Svensson et al.

Amano, Kungsgatan 1, Örebro. Närproducerade, ekologiska råvaror och viner av högsta kvalité brinner vi för. Färsk gös direkt från Hjälmaren, lokalt plockad svamp och oljor från Julita är bara ett par exempel.

CGTase derived from Bacillus macerans was obtained as a kind gift from Amano Enzyme, Inc., Nagoya, Japan, in a stock solution that was used as supplied and stored at 5 °C. Water was purified on a Merck Millipore Synergy UV water purification system prior to use. Cyclodextrin glucanotransferase from B. macerans (CGTase, EC 2.4.1.19) with activity of 600 U/ml was purchased from Amano Enzyme Inc., Japan. Water soluble potato starch was purchased from Sigma, Malaysia.

Existen tres CD con Estas moléculas, deberán tener un tamaño compatible con la cavidad interna de la CD, . of cyclodextrin glucosyltransferase (CGTase, EC 2.4.1.19) on hydrolyzed starch.